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Journal of Wildlife Diseases, 9(2), 1973, pp. 120-124
© Wildlife Disease Association  1973
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NEUTRALIZATION TESTS OF INFECTIOUS PANCREATIC NECROSIS VIRUS WITH POLYVALENT ANTISERUM

JOSEPH C. LIENTZ 1 and JERRY E. SPRINGER 2

1 U.S. Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife Fish Cultural Development Center, Bozeman, Montana, U.S.A.
2 Willow Beach National Fish Hatchery, Willow Beach, Arizona, U.S.A.

Forty-two isolates of infectious pancreatic necrosis virus were cloned by plaquing, and the clones were used in neutralization tests with a 5-strain polyvalent serum. Seventy-six percent of the strains tested were completely neutralized, 19 percent were partially neutralized and 5 percent were not neutralized. Data provide a measure of the antiserum's diagnostic effectiveness and indicate that at least one more antigenic type should be included in preparation of a definitive polyvalent serum.

Submitted on May 22, 1972






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