JWD
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Wildlife Diseases, 45(4), 2009, pp. 1021-1029
© Wildlife Disease Association  2009
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Arenas-Gamboa, A. M.
Right arrow Articles by Rice-Ficht, A. C.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arenas-Gamboa, A. M.
Right arrow Articles by Rice-Ficht, A. C.

ORAL VACCINATION WITH MICROENCAPSULED STRAIN 19 VACCINE CONFERS ENHANCED PROTECTION AGAINST BRUCELLA ABORTUS STRAIN 2308 CHALLENGE IN RED DEER (CERVUS ELAPHUS ELAPHUS)

 Angela M. Arenas-Gamboa1,2,4, Thomas A. Ficht1, Donald S. Davis1, Philip H. Elzer3, Melissa Kahl-McDonagh1, Alfredo Wong-Gonzalez1 and Allison C. Rice-Ficht1,2

1 Department of Veterinary Pathobiology, Texas A&M University and Texas Agricultural Experiment Station, College Station, Texas 77845-1114, USA
2 Department of Molecular and Cellular Medicine, Texas A&M Health Science Center, College Station, Texas 77845-1114, USA
3 Department of Veterinary Science, Louisiana State University, Ag Center Baton Rouge, Louisiana 70803, USA
4 Corresponding author (email: aarenas{at}cvm.tamu.edu)

ABSTRACT:   Bison (Bison bison) and elk (Cervus elaphus nelsoni) in the Greater Yellowstone Area (GYA), USA, are infected with Brucella abortus, the causative agent of bovine brucellosis, and they serve as a wildlife reservoir for the disease. Bovine brucellosis recently has been transmitted from infected elk to cattle in Montana, Wyoming, and Idaho and has resulted in their loss of brucellosis-free status. An efficacious Brucella vaccine with a delivery system suitable for wildlife would be a valuable tool in a disease prevention and control program. We evaluated Strain 19 (S19) in a sustained release vehicle consisting of alginate microspheres containing live vaccine. In a challenge study using red deer (Cervus elaphus elaphus) as a model for elk, alginate, a naturally occurring polymer combined with a protein of Fasciola hepatica vitelline protein B was used to microencapsulate S19. Red deer were orally or subcutaneously immunized with 1.5x1010 colony-forming units (CFUs) using microencapsulated S19. Humoral and cellular profiles were analyzed bimonthly throughout the study. The vaccinated red deer and nonvaccinated controls were challenged 1 yr postimmunization conjunctivally with 1x109 CFUs of B. abortus strain 2308. Red deer vaccinated with oral microencapsulated S19 had a statistically significant lower bacterial tissue load compared with controls. These data indicate for the first time that protection against Brucella-challenge can be achieved by combining a commonly used vaccine with a novel oral delivery system such as alginate-vitelline protein B microencapsulation. This system is a potential improvement for efficacious Brucella-vaccine delivery to wildlife in the GYA.
  Key words:  Brucella abortus, brucellosis, Cervus elaphus, encapsulation, red deer, vaccine.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2009 by the Wildlife Disease Association.