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¹ School of Veterinary Science, Seddon Building, Slip Road, The University of Queensland, QLD, 4072, Australia
² Molecular Parasitology Laboratory, Queensland Institute for Medical Research, 300 Herston Road, Brisbane, QLD, 4029, Australia
³ Corresponding author (email: t.barnes{at}uq.edu.au)

ABSTRACT:   This study examined the suitability of immunoblot assays using antigen B from hydatid cyst fluid (HCF) and the recombinant protein EpC1 to estimate the prevalence of hydatid infection in threatened small macropodid populations. When HCF antigen B was used on sera from 29 wild eastern grey kangaroos (Macropus giganteus) examined for the presence of hydatid cysts at necropsy (17 positive and 12 negative), and from five captive-bred macropodids, the sensitivity and specificity were 88% and 41%, respectively. Following optimization, the use of rEpC1 on sera from 51 wild eastern grey kangaroos (48 positive and 3 negative at necropsy), and from 9 captive bred macropodids, resulted in sensitivity and specificity of 48% and 92% respectively.
  Key words:  Echinococcus granulosus, hydatid, immunoblot, immunodiagnosis, macropodid.