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¹ Department of Microbiology and Immunology, University of British Colombia, 6174 University Blvd., Vancouver, British Columbia V6T 1Z3, Canada;
² Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada;
³ Provincial Laboratory for Public Health, 3030 Hospital Drive N.W., Calgary, Alberta T2N 4W4, Canada;
⁴ Wildlife Division, Northwest Territories Environment and Natural Resources, 5102 50th Ave., Yellowknife, Northwest Territory X1A 3S8, Canada
⁵ Corresponding author (email: murray.woodbury{at}usask.ca)

ABSTRACT:   Nested polymerase chain reaction (PCR) using the Mycobacterium avium subspecies paratuberculosis (Map)–specific region, locus 251, was used as a screening tool for the detection of Map DNA in fecal samples from northern Canadian bison herds. Further characterization of positive samples (26/835) was performed because Map DNA was found without signs of disease. Strain typing, using PCR-Restriction endonucleas assay (REA), was limited to two samples but revealed that the samples corresponded to a cattle-related strain and a sheep-related strain. Sequencing of part of the IS1311 region from the two samples revealed a unique three base-pair region, which is only found within the northern Canadian bison isolates.
  Key words:  Bison, Mycobacterium paratuberculosis, PCR, PCR-REA.