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¹ Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA;
² Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13005 Ciudad Real, Spain;
³ Montana Fish, Wildlife and Parks, 1400 S. 19th St., Bozeman, Montana 59718, USA;
⁴ Montana Conservation Science Institute, Ltd., 5200 Upper Miller Creek Road, Missoula, Montana 59803, USA;
⁵ Animal Population Health Institute, Veterinary Teaching Hospital, Colorado State University, 300 West Drake, Fort Collins, Colorado 80523, USA
⁶ Corresponding author (email: jose_delafuente{at}yahoo.com or djose{at}cvm.okstate.edu)

ABSTRACT:   Wildlife reservoir species and genetic diversity of Anaplasma ovis (Rickettsiales: Anaplasmataceae) have been poorly characterized. Bighorn sheep (Ovis canadensis), captured in Montana from December 2004 to January 2005, were tested for antibodies to Anaplasma spp.; the presence of A. ovis was determined by the characterization of major surface protein msp4 sequences. Anaplasma antibodies were detected in 25/180 (14%) sampled bighorn sheep and A. ovis msp4 sequences were amplified by polymerase chain reaction (PCR) and sequenced from 9/23 (39%) of seropositive animals. All animals were negative by PCR for the related pathogens, Anaplasma phagocytophilum and Anaplasma marginale. All msp4 sequences identified in the bighorn sheep were identical and corresponded to a single A. ovis genotype that was identical to a sheep isolate reported previously from Idaho. The finding of a single genotype of A. ovis in this wild herd of bighorn sheep was in contrast to the genetic diversity reported for A. marginale in cattle herds in the western United States and worldwide. These results demonstrated that bighorn sheep may be a wildlife reservoir of A. ovis in Montana.
  Key words:  Anaplasma ovis, anaplasmosis, bighorn sheep, major surface protein, msp4, reservoir host.

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				J. C. Haigh, V. Gerwing, J. Erdenebaatar, and J. E. Hill A NOVEL CLINICAL SYNDROME AND DETECTION OF ANAPLASMA OVIS IN MONGOLIAN REINDEER (RANGIFER TARANDUS) J. Wildl. Dis., July 1, 2008; 44(3): 569 - 577. [Abstract] [Full Text] [PDF]