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¹ Institute for Zoo and Wildlife Research Berlin, Alfred-Kowalke-Strasse 17, P.O. Box 601103, 10252 Berlin, Germany;
² Friedrich-Loeffler-Institute, Boddenblick 5a, 17493 Greifswald-Insel Riems, Germany;
³ Forschungsstelle für Jagdkunde und Wildschadenverhütung der Landesanstalt für Ökologie, Bodenordnung und Forsten Nordrhein-Westfalen, Forsthaus Hardt, Pützchens Chaussee 228, 53229 Bonn, Germany;
⁴ Institute of Wildlife Research at the School of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany
⁵ Corresponding author (email: froelich{at}izw-berlin.de)

ABSTRACT:   Blood samples (n = 223) of free-ranging roe deer (Capreolus capreolus) were collected from selected hunting grounds in Germany between October 2001 and October 2002. Samples originated from Lower Saxony (n = 43) and North-Rhine Westphalia (n = 108) within a 20-km area ("cordon") cordoned off along the border of The Netherlands. This is adjacent to the area of a foot-and-mouth disease outbreak that occurred between 21 March and 22 April 2001 in The Netherlands. Negative control samples were taken from northern Germany (Schleswig-Holstein, n = 72). Two different enzyme-linked immunosorbent assays (ELISAs) were used for the detection of antibodies against foot-and-mouth disease virus (FMDV) serotype O strain Manisa. To confirm ELISA-positive results, a virus neutralization test was performed. All samples tested negative for antibodies against FMDV. These results suggest that FMDV was not transmitted to free-ranging roe deer living in parts of Germany adjacent to the area affected by the 2001 foot-and-mouth disease outbreak in The Netherlands.
  Key words:  Capreolus capreolus, ELISA, enzyme-linked immunosorbent assay, foot-and-mouth disease virus, Germany, roe deer, serologic survey.

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