| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
SHORT COMMUNICATION |
1 Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine;
2 D. B. Warnell School of Forest Resources, The University of Georgia, Athens, Georgia 30602, USA;
3 Johne’s Testing Center, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin 53706, USA
5 Corresponding author (email: rdavidso{at}vet.uga.edu)
ABSTRACT:
From July 1998 through October 2002, radiometric culture (ileocecal lymph node, mesenteric lymph node, and feces) and serologic testing by enzyme-linked immunosorbent assay (ELISA) were used to survey white-tailed deer (Odocoileus virginianus) from the southeastern United States for infection by Mycobacterium avium subsp. paratuberculosis (Mptb), the causative agent of paratuberculosis (Johne’s disease). Mycobacterium avium subsp. paratuberculosis was isolated from the ileocecal lymph node of one of 313 deer (0.3%) originating from 63 populations in Alabama, Arkansas, Florida, Georgia, Kentucky, Louisiana, Maryland, Mississippi, North Carolina, South Carolina, Tennessee, and West Virginia (USA). Six deer (2%), all from different populations, had ELISA results above a 0.25 sample-to-positive cutoff value, but none of the ELISA reactors originated from the population from which the single Mptb isolation was made. These six deer were seronegative when tested by agar gel immunodiffusion (AGID). Collectively, these data indicate that white-tailed deer currently do not constitute a broad regional reservoir for Mptb; however, further study is warranted to clarify the significance, if any, of infected deer to the epizootiology of paratuberculosis on a local scale. Adaptation and validation of an ELISA or another serologic assay for use with deer and other wildlife would markedly enhance Mptb surveillance among wild populations and would be a powerful tool for gaining information on the role of wild species in epidemiology of paratuberculosis.
Key words: ELISA, Johne’s disease, Mycobacterium avium subsp. paratuberculosis, Odocoileus virginianus, radiometric culture, survey, United States, white-tailed deer.
4 Current address: Wildlife Health Associates Inc., PO Box 109, Dillon, Montana 59725, USA
This article has been cited by other articles:
|
|
 |
|
  M. V. Palmer, J. R. Stabel, W. R. Waters, J. P. Bannantine, and J. M. Miller EXPERIMENTAL INFECTION OF WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS) WITH MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS J. Wildl. Dis., October 1, 2007; 43(4): 597 - 608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Crawford, M. H. Ziccardi, B. J. Gonzales, L. M. Woods, J. K. Fischer, E. J. B. Manning, and J. A. K. Mazet MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS AND MYCOBACTERIUM AVIUM SUBSP. AVIUM INFECTIONS IN A TULE ELK (CERVUS ELAPHUS NANNODES) HERD J. Wildl. Dis., October 1, 2006; 42(4): 715 - 723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. T. Griffin, E. Spittle, C. R. Rodgers, S. Liggett, M. Cooper, D. Bakker, and J. P. Bannantine Immunoglobulin G1 Enzyme-Linked Immunosorbent Assay for Diagnosis of Johne's Disease in Red Deer (Cervus elaphus) Clin. Vaccine Immunol., December 1, 2005; 12(12): 1401 - 1409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Godfroid, C. Delcorps, L. M. Irenge, K. Walravens, S. Marche, and J.-L. Gala Definitive Differentiation between Single and Mixed Mycobacterial Infections in Red Deer (Cervus elaphus) by a Combination of Duplex Amplification of p34 and f57 Sequences and Hpy188I Enzymatic Restriction of Duplex Amplicons J. Clin. Microbiol., September 1, 2005; 43(9): 4640 - 4648. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
