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Journal of Wildlife Diseases, 40(1), 2004, pp. 99-102
© Wildlife Disease Association  2004
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SHORT COMMUNICATION

Detection of Mustelid Herpesvirus-1 Infected European Badgers (Meles meles) in the British Isles

Donald P. King1,4,5, Naledi Mutukwa1, Sandrine Lesellier2, Chris Cheeseman3, Mark A. Chambers2 and Malcolm Banks1

1 Departments of Virology and
2 Bacterial Diseases, Veterinary Laboratories Agency, Woodham Lane, Addlestone, Surrey KT15 3NB, UK;
3 Wildlife Disease Ecology Team, Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
5 Corresponding author (email: donald.king{at}bbsrc.ac.uk)

ABSTRACT:   The aim of this study was to assess the frequency of mustelid herpesvirus-1 (MusHV-1) infection in free-ranging badgers (Meles meles) in the British Isles. A polymerase chain reaction assay was developed that detected MusHV-1 DNA in 95% (18/19) and 100% (10/10) of anticoagulant-treated blood samples collected from free-ranging badgers sampled in the southwest of England and the Republic of Ireland, respectively. An indirect immunoassay was also developed to detect MusHV-1–specific immunoglobulin-G in serum samples. Using an arbitrary cutoff of twice the optical density obtained with a virus-negative preparation, 32.7% (36/110) of sera sampled from badgers were positive. The conclusion drawn from these data is that infection with MusHV-1 is common among free-ranging badgers in the British Isles.
  Key words:  Badger, herpesvirus, PCR, serology.

FOOTNOTES

4 Current address: Department for Exotic Disease Control, Institute for Animal Health, Ash Road, Pirbright GU24 0NF, UK






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