JWD Your personal alerts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Wildlife Diseases, 39(1), 2003, pp. 125-135
© Wildlife Disease Association  2003
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Samuel, M.
Right arrow Articles by Johnson, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Samuel, M.
Right arrow Articles by Johnson, W.

Comparison of methods to detect Pasteurella multocida in carrier waterfowl

MD Samuel, DJ Shadduck, DR Goldberg, and WP Johnson


ABSTRACT

We conducted laboratory challenge trials using mallard ducks (Anas platyrhynchos) to compare methods for detecting carriers of Pasteurella multocida, the bacterium that causes avian cholera, in wild birds. Birds that survived the initial infection were euthanized at 2-4 wk intervals up to 14 wk post challenge. Isolates of P. multocida were obtained at necropsy from 23% of the birds that survived initial infection. We found that swab samples (oral, cloacal, nasal, eye, and leg joint) were most effective for detecting carrier birds up to 14 wk post infection. No detectable differences in isolation were observed for samples stored in either 10% dimethysulfoxide or brain heart infusion broth. The frequency of detecting carriers in our challenge trials appeared to be related to mortality rates observed during the trial, but was not related to a number of other factors including time after challenge, time delays in collecting tissues postmortem, and route of infection. In our trials, there was little association between antibody levels and carrier status. We concluded that swabs samples collected from recently dead birds, stored in liquid nitrogen, and processed using selective broth provide a feasible field method for detecting P. multocida carriers in wild waterfowl.


This article has been cited by other articles:


Home page
 J Wildl DisHome page
D. S. Blehert, K. L. Jefferson, D. M. Heisey, M. D. Samuel, B. M. Berlowski, and D. J. Shadduck
USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS TO DIFFERENTIATE ISOLATES OF PASTEURELLA MULTOCIDA SEROTYPE 1
J. Wildl. Dis., April 1, 2008; 44(2): 209 - 225.
[Abstract] [Full Text] [PDF]

Home page
 J Wildl DisHome page
J. A. Blanchong, M. D. Samuel, D. R. Goldberg, D. J. Shadduck, and M. A. Lehr
Persistence of pasteurella multocida in wetlands following avian cholera outbreaks.
J. Wildl. Dis., January 1, 2006; 42(1): 33 - 39.
[Abstract] [Full Text] [PDF]

Home page
 J Wildl DisHome page
M. D. Samuel, D. J. Shadduck, and D. R. Goldberg
AVIAN CHOLERA EXPOSURE AND CARRIERS IN GREATER WHITE-FRONTED GEESE BREEDING IN ALASKA, USA
J. Wildl. Dis., July 1, 2005; 41(3): 498 - 502.
[Abstract] [Full Text] [PDF]

Home page
 J Wildl DisHome page
M. D. Samuel, D. J. Shadduck, D. R. Goldberg, and W. P. Johnson
AVIAN CHOLERA IN WATERFOWL: THE ROLE OF LESSER SNOW AND ROSS'S GEESE AS DISEASE CARRIERS IN THE PLAYA LAKES REGION
J. Wildl. Dis., January 1, 2005; 41(1): 48 - 57.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Wildlife Disease Association.