Nine species of ectoparasites (4 Acari, 2 Mallophaga, 1 Anoplura, 1 Diptera, and 1 Siphonaptera) were recovered from 126 white-tailed deer collected in northern, central, and southern Mississippi. Intensity and prevalence of adults of Ixodes scapularis and larvae, nymphs, and adults of Amblyomma americanum varied significantly over collection periods, but not between host sexes. Lipoptena mazamae occurred on deer from only one study area. Although individual deer were heavily parasitized by Tricholipeurus parallelus and T. lipeuroides, their prevalence was limited. Hoplopsyllus sp., Solenopotes sp., Amblyomma maculatum, and Dermacentor albipictus had prevalences of less than 10% and were not tested for area, host sex, and seasonal effects. The potential pathogenicity of these ectoparasite species are related to white-tailed deer in Mississippi.