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Journal of Wildlife Diseases, 21(3), 1985, pp. 211-218
© Wildlife Disease Association  1985
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Detection of Vibrio anguillarum antigen by the dot blot assay

RC Cipriano, JB Pyle, CE Starliper, and SW Pyle


ABSTRACT

The dot blot assay, modified and adapted for detection of antigens from Vibrio anguillarum in fish tissues, was specific for V. anguillarum and did not react with antigens of V. ordalii, Pseudomonas sp., or Yersinia ruckeri. The blot assay enabled detection of as little as 2.3 ng of a mixture of protein antigens obtained from cell-free extracts of V. anguillarum; it was about 100 times more sensitive than either the indirect fluorescent antibody technique or bacterial isolation for detecting V. anguillarum in fish tissues.


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N. Bisharat, C. Amaro, B. Fouz, A. Llorens, and D. I. Cohen
Serological and molecular characteristics of Vibrio vulnificus biotype 3: evidence for high clonality
Microbiology, March 1, 2007; 153(3): 847 - 856.
[Abstract] [Full Text] [PDF]


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Copyright © 1985 by the Wildlife Disease Association.