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Twenty mule deer fawns (Odocoileus hemionus) were removed from their dams 48 h after birth, and hand-reared. Methods for monitoring their immune capability are described. Passive humoral immunity was determined by serum protein electrophoresis. Active humoral immunity following Clostridium toxoid vaccination was determined by immunodiffusion. Cell-mediated immunity was assayed using contact sensitization to 1-nitro, 2,4-dichlorobenzene (DNCB).
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